Mycoplasma Hyopneumoniae Serology
The serology services laboratory is pleased to announce that
an ELISA test for antibodies against Mycoplasmahyopneumoniaehas
been developed by Dr. Kanitz at the PurdueADDL and was brought
"on line" December 1st
During the last year or so, the ADDL has received many requests
from swine practitioners for M.hyo.serology, to be
used along with the currently available PRRSV and SIV tests
as an aid in management of swine respiratory disease. This
new test is in answer to these requests.
Since M.hyo.infections are nearly ubiquitous in the
general swine population, it was thought that a simple positive/negative
serology result would be less than adequate. Some sort of
quantification would be desirable in order to assess the relative
immune status of groups of pigs and to determine when and
where infections are occurring. To this end the new M.
hyo.ELISA test has been set up and calibrated to give
numerical results similar to those of the PRRSV ELISA test.
Results will be reported as S/P ratios as well as positive/negative.
As with the PRRSV test, the S/P cut off point has been set
at 0.40.
Although the new M. hyo. ELISA test has been developed
using standard, accepted ELISA technology and high quality
reagents, it has not yet been fully evaluated for sensitivity
and specificity. Results accumulated to date have shown clear-cut
differences between positive field samples and a large bank
of known (or presumed) negative field and experimental samples.
The M. hyo. ELISA will be priced as a standard mammalian
serology test; that is, $2.00 per test for Indiana
swine and $4.00 for out-of-state and research submissions.
Testing schedules and turn around times have not yet been
worked out. They will be partially dependent on the number
and frequency of serums received for testing.
- by Charles L.Kanitz, DVM,
MS, PhD Chief of Virology
and Serology, ADDL
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